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Isolation:
Isolation date: April, 1952
Virus Susceptibility:
Vesicular stomatitis virus
Human poliovirus 1
Antigen Expression:
H-2k
Cytogenetic Analysis:
modal number = 41; range = 38 to 86.
Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with satellites.
Age:
newborn
Gender:
male
Comments:
Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Subculturing:
Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks.
A standard trypsinizaton may be used if desired.
The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination.
It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 3 times per week
Preservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References:
22167: Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690 26070: Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026 26074: . . J. Natl. Cancer Inst. 23: 823, 1959. 26076: . . J. Natl. Cancer Inst. 27: 29, 1961. 26078: Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589 26079: . . Fed. Proc. 17: 967, 1958. 26081: Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338
