| MMQ |
| University of Virginia Patent Foundation |
| Biosafety Level: | 1 |
| frozen |
| See Propagation |
| suspension |
| Rattus norvegicus (rat) |
| epithelial
|
| Organ: pituitary
Disease: tumor |
| prolactin |
| In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
| dopamine |
| No |
| 5 days |
| ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: - fetal bovine serum to a final concentration of 2.5%
- horse serum to a final concentration of 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
DGrowth Conditions: The line grows as clumps of cells in suspension.
|
| Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 days |
| Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| 24 to 48 hrs |
| Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
Cell culture tested DMSO:ATCC 4-X |
| 22090: MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996 |
